Table 1

T and B Cell Subpopulations in Ship/−Rag/− Chimeric Mice

Ship+/−Rag−/−Ship−/−Rag−/−
Thymus
 Total cell number (× 107)     8 ± 0.6  8.6 ± 0.9
 CD4+CD8+ (% ± SEM)81.5 ± 4.1 76.6 ± 4.9
 CD4+CD813.3 ± 3.2 17.6 ± 3.9
 CD4CD8+ 2.3 ± 1.0  2.8 ± 0.8
 CD4CD8    2.9± 0.2  3.1 ± 0.4
Lymph nodes
 Total cell number (× 107) 2.4 ± 0.6      2 ± 0.5
 CD4+CD8 (% ± SEM)58.3 ± 3.168.5 ± 3.5
 CD4CD8+17.8 ± 2.8 13.4 ± 2.5
Spleen
 Total cell number (× 107) 6.5 ± 0.4     11 ± 1.2
 CD4+CD8 (% ± SEM)29.2 ± 4.947.4 ± 3.1
 CD4CD8+13.8 ± 2.0  9.4 ± 4.8
 B220+sIgM+    36 ± 5.5     27 ± 4.5
 sIgD+sIgMhi10.7 ± 2.0 4.7 ± 1.7
 sIgDhisIgMlo24.2 ± 4.1     20 ± 3.5
Bone marrow
 Total cell number (× 107) 1.2 ± 0.3  1.4 ± 0.3
 B220+CD43+ (% ± SEM) 8.5 ± 3.3  9.8 ± 2.4
 BD220+CD4345.4 ± 4.619.1 ± 2.9
 B220+CD25+IgM 7.1 ± 1.4 4.5 ± 1.0
 B220loHSAhi26.4 ± 4.2 25.3 ± 2.6
 B220+HSAlo    16 ± 3.1 4.9 ± 1.2
 B220+sIgM+16.4 ± 2.1 8.7 ± 2.5
  • Cells from Ship+/− (n = 5) and Ship−/− (n = 7) chimeric mice were stained with the indicated antibodies, and populations were determined using a FACScan®. Bold numbers, Statistically significant differences between Ship+/− and Ship−/− subpopulations.