Table 2

Differential Counts of CFU Assays

Growth factorGenotypeSegmented neutrophilsMacrophageImmature
%%%
GM-CSF+/−53.8 ± 13.8 6.5 ± 3.539.8 ± 10.2
−/−20.7 ± 2.3 7.2 ± 1.972.2 ± 0.8
IL-3+/−51.0 ± 6.211.7 ± 1.540.7 ± 4.9
−/−17.3 ± 7.0 7.2 ± 3.375.5 ± 10.3
IL-6 + soluble IL-6 receptor+/−38.7 ± 8.433.0 ± 6.328.3 ± 2.3
−/− 1.2 ± 0.348.3 ± 8.750.5 ± 9.0
G-CSF + G-CSF receptor+/−43.0 ± 14.723.1 ± 11.734.3 ± 3.3
−/−26.0 ± 5.422.3 ± 7.651.7 ± 3.3
G-CSF + IL-6 + soluble IL-6 receptor+/−46.1 ± 9.431.6 ± 11.422.3 ± 4.6
−/−16.5 ± 6.437.2 ± 6.846.3 ± 8.1
G-CSF + IL-6 + G-CSF receptor + soluble IL-6 receptor+/−48.7 ± 8.733.1 ± 2.518.1 ± 11.4
−/−40.6 ± 5.533.3 ± 7.022.5 ± 8.3
  • Using the colonies generated as described in the legend to Table 1, percentages of different types of cells were determined by counting 200–300 cells from each slide. Each number was derived from a total of 3 to 4 individual animals, and at least 16 colonies for CFU-G and CFU-IL6. Immature cells include myeloblasts and metamyelocytes; segmented neutrophils include band forms.