Table 1

OVA-induced Leukocyte Infiltration in the Airways after Chemokine Blockage

Chemokine blockageLeukocyte subtype
MacrophagesMonocytesT lymphocytesEosinophils
Day 15Day 21Day 15Day 21Day 15Day 21Day 15Day 21
Control (total(180 ± 31) × 103(122 ± 12) × 103(38 ± 2) × 103(25 ± 3) × 103(27 ± 5) × 103(130 ± 22) × 103(80 ± 17) × 103(870 ± 92) × 103
 numbers)
αEotaxin017 ± 40 7 ± 1 9 ± 221 ± 4      27 ± 3        60 ± 7
αMCP-5        12 ± 311 ± 2       66 ± 811 ± 322 ± 50      76 ± 3        21 ± 8
Met-RANTES         9 ± 117 ± 1       26 ± 444 ± 880 ± 295 ± 460 ± 10        91 ± 2
αMIP-1α0     2 ± 0.300000        23 ± 2
αMCP-145 ± 1058 ± 378 ± 1153 ± 591 ± 389 ± 6      86 ± 878 ± 12
  • Percentage of reduction in the number of leukocytes in the BAL fluid of OVA-treated mice after chemokine blockage. 30 min before each intranasal antigen administration, eotaxin, MCP-5, RANTES, or MCP-1 was neutralized as described in Materials and Methods. BAL fluid was obtained 3 h after OVA treatment on day 15 or 21, and the number of the different cell types was determined. Total numbers of macrophages, monocytes, T lymphocytes, and eosinophils present in the BAL fluid of OVA plus Ab control–treated mice are shown. Values are given as percentages referred to the OVA plus Ab control–treated mice, which are considered to be 100%.