Table 3

Increase in TUNEL+ Mac-1+ Cells from Tissue of Mice Infected with Wild-type Y. pseudotuberculosis Bacteria

TissueBacterial strainPercentage of gate R1, TUNEL+ cells ± SE*P value‡Percentage of Mac-1+ ± SE§P value¶Mean CFU recovered
Day 4
MLNYPIIIpVY 9.85 ± 4.20 3.38 ± 0.362.42 × 103
MLNYPIIIpyopJ 2.14 ± 0.78 2.62 ± 0.412.48 × 103
SpleenYPIIIpYV12.08 ± 4.12 4.97 ± 1.385.13 × 102
SpleenYPIIIpyopJ 2.17 ± 0.47 7.10 ± 0.923.13 × 102
Day 5
MLNYPIIIpYV21.41 ± 4.960.0013 3.95 ± 0.250.01614.45 × 103
MLNYPIIIpyopJ 2.01 ± 0.37 3.74 ± 0.360.04341.33 × 103
SpleenYPIIIpYV27.82 ± 5.400.0002 5.86 ± 1.053.94 × 103
SpleenYPIIIpyopJ 2.14 ± 0.3312.32 ± 1.420.01672.25 × 103
MLNUninfected 3.45 ± 1.41 1.91 ± 0.89
SpleenUninfected 1.74 ± 0.55 5.96 ± 0.92
  • *  Mac-1 (M1/70) antibody conjugated to biotin was used to stain single cell suspensions. Streptavidin-PE was detected in FL-2. Cells within gate R1 were analyzed to obtain the percentage of Mac-1+ cells with a forward scatter in the range of monocytes or macrophages that were TUNEL positive. n = 9 mice per inoculum/day, n = 5 uninfected mice.  

  • P values are from a t test comparing percentage of gate R1, Mac-1+ cells that were also TUNEL positive obtained for YPIIIpYV-infected tissue compared to values obtained for YPIIIpyopJ-infected tissue. Values only given when P ≤ 0.0500.  

  • §  The relative percentage of Mac-1+ cells was obtained by gating on cells positive in FL-2. Cell debris was gated out.  

  • P values are from a t test comparing percentage Mac-1+ cells obtained for either YPIIIpYV-infected tissue or YPIIIpyopJ-infected tissue to values obtained for uninfected tissue. Values only given when P ≤ 0.0500.