Table 2

Surface Phenotype of Fresh and GM-CSF–exposed RTDCs*

MarkermAbFreshPlus GM-CSF
Percentage frequenciesMFIPercentage frequenciesMFI
CD4W3/25      29.7     4.5   2.9     2.7
CD8OX8       2.0     3.6   2.5     6.0
CD11aWT.1      13.9     3.7  19.4     7.2
CD11b/cOX42       3.3     3.1       10.8     6.8
CD45OX1       5.9     3.3   8.0     3.0
CD541A-29       8.9     6.3   4.1 9.9
CD80/86CTLA-4 Ig        0.8 2.2        54.838.6
MHC class IOX27   18.7 5.4    62.010.2
MHC class IIOX610011.510055.1
  • *  Lung digests were depleted of mφ and B cells as described in Materials and Methods and labeled with the indicated mAbs and GAM-PE (or CTLA-4 Ig and GAH-PE) followed by OX6-FITC. Percentage frequencies and mean fluorescence intensities (MFI) are shown for 5 × 103 OX6+ gated RTDCs using positive analysis regions set on isotype-matched controls.