Table 1

Splenocytes from μMT KO Mice but not from CD4 KO Mice Confer Protection from EAE

Treatment*nMice without EAE‡Maximum EAETotal recovery§Final EAE‖CD8+ cells reconstitution¶γ/δ+ cells reconstitution¶
3 × 106 CD4 KO70/72.4 ± 1.361/71.5 ± 1.7NDND
2 × 106 CD4 KO50/5  2.4 ± 0.50/51.9 ± 0.50.20.03
3 × 106 μMT KO32/3  0.7 ± 1.20/10.2 ± 0.3NDND
2 × 106 μMT KO52/5  0.8 ± 0.82/30.2 ± 0.30.40.02
PBS40/4  2.8 ± 1.50/42.5 ± 1.7NDND
  • T/R mice (<31 d old) transferred with different numbers of CD4 KO or μMT KO splenocytes, or injected with PBS. Mice were observed for a period of 140 d and EAE was scored as described in Materials and Methods.  

  • *  Injected cells correspond to the number of total CD8+ cells.  

  •  Observed mice with no signs of EAE.  

  • §  Observed mice that recovered completely from EAE.  

  •  Average EAE score at the end of the experiment.  

  •  Number of CD8+ or γ/δ+ cells per one anti-MBP-specific TCR transgenic cell after 140 d of treatment.