Table 1

Effects of TGF-β1 on CD1a, E-cadherin, CLA, and Lag Expression

day 6
day 0GM-CSF + IL-4GM-CSF + IL-4 + anti–TGF-βGM-CSF + IL-4 + TGF-β1
Cell number (× 105/ml107.225 ± 0.66.35 ± 0.87.25 ± 0.5
% CD1a-positive cells ± SD<189.25 ± 3    91 ± 3    93 ± 4
% E-cad-positive cells ± SD<1 11.1 ± 6 6.2 ± 5    82 ± 8
% CLA-positive cellsND<10<1080–90
% Lag-positive cells 00ND20–40
  • Anti–TGF-β1 blocking antibody was used at a concentration (20 μg/ml) that inhibits as high as 10 ng of recombinant TGF-β1. CD1a and E-cadherin expressions were determined by flow cytometry analysis; CLA and Lag expressions were determined by immunocytochemistry. Results are the mean (±SD) of four experiments.