Table 1

Effect of Anti-ST2L Antibody on T Cells In Vitro

Treatment of cellsTh1 (X4) percent viabilityTh2 (X12) percent viability
Anti-ST2L + C′85.2 ± 2.1     9.7 ± 0.5*
Anti-ST2L alone94.9 ± 3.595.2 ± 4.6
Normal IgG + C′95.9 ± 2.794.6 ± 3.3
Normal IgG alone95.0 ± 2.997.2 ± 5.8
Medium alone96.2 ± 3.595.8 ± 3.9
  • Th1s and Th2s were incubated with 400 μg/ml of antibody and then with or without complement (C′). The cell viability was determined microscopically. At this concentration, in the absence of complement, anti-ST2L antibody, but not normal IgG, also partially suppressed the proliferation and cytokine production by Th2s in vitro in response to specific antigen and antigen-presenting cells. The antibody did not affect the proliferation of Th1s (data not shown). Data are mean ± SEM, n = 3.  

  • *P <0.001 compared with medium control. Similar results were obtained for three other Th1 and Th2 clones.