Table 1

cDNA and Germline Clones Isolated

VH4 gene alleles isolated*cDNA clones with ins/delTotal cDNA clones isolatedGermline clones isolated‡
VH4–392113 7
VH4–312 49 8
VH4–591 45 4
VH4–341 8711
VH4–34 related0  0 4
VH4–55 pseudogene§0  012
VH4–55-related pseudogene§0  0 3
VH4–040 17 7
VH4–04-related pseudogene§0  0 2
VH4–610 25 7
New VH4 gene0 33 3
VH4–04B0 72 1
VH4–280  0 1
  • *  Nomenclature based on Matsuda and Honjo (37).  

  •  Nine unusual isolates were also cloned consisting of hybrids of two of the indicated genes, resumably do to PCR artifact. None of these artifacts were altered in size or resembled any of the insertion or deletion events observed.  

  • §  Pseudogenes contain stop codons or frameshift mutations and are not expressed.  

  • **  Newly identified VH4 gene is most closely related to VH4–04.