Table 1

Kinetics of BM Cytology of C57BL/6, P0/0, and P0/0 × TCR Mice Infected with LCMV

GenotypeDays* after infectionCell type (%)§
Megakaryocyte‡ErythroblastBlasts promyelocyteMyelocyte metamyelocyte bandPolymorphMonocyteLymphocyte
P0/0Uninfected12–2435.5 ± 1.0 9.5 ± 1.836.5 ± 2.8  5.0 ± 1.5 0.5 ± 0.313.0 ± 1.4
C57BL/6Day 6 6–10 9.5 ± 0.727.5 ± 1.431.5 ± 1.4  9.5 ± 2.0 5.5 ± 0.816.5 ± 2.6
P0/0 6–1211.5 ± 0.428.5 ± 1.427.0 ± 1.8 10.0 ± 2.0 3.0 ± 0.520.0 ± 1.3
P0/0 × TCR4–8 4.0 ± 0.227.0 ± 1.332.0 ± 1.1  7.0 ± 0.8 3.5 ± 1.526.5 ± 2.8
C57BL/6Day 8 6–10 4.5 ± 0.623.0 ± 2.037.5 ± 0.8 18.0 ± 1.0 6.0 ± 1.011.0 ± 2.3
P0/0 5–10 7.0 ± 0.340.5 ± 2.829.0 ± 2.0  3.0 ± 0.1 6.5 ± 0.514.0 ± 1.8
P0/0 × TCR     0 2.5 ± 0.431.5 ± 3.3 8.0 ± 0.31.0 ± 0.3 9.5 ± 4.547.5 ± 2.0
C57BL/6Day 14 8–1422.0 ± 1.516.5 ± 0.820.5 ± 2.2 31.0 ± 3.0 1.5 ± 0.8 9.0 ± 0.8
P0/0∥0–3 2.0 ± 0.510.0 ± 1.028.5 ± 9.2  5.0 ± 1.024.0 ± 1.529.5 ± 0.3
  • *  Mice (8–12 wk) of different genotypes (three mice per group) were infected with LCMV (2 × 102 PFU). At the time points indicated, BM smears were stained with May-Grünwald/Giemsa, chloracetate esterase, α-naphthyl butyrate esterase, and Sudan black B. Differential counts were obtained microscopically by counting 200 or more cells.  

  •  Numbers represent megakaryocytes per visual field at a magnification of 80. They were determined semiquantitatively by counting at least four visual fields.  

  • §  Data are means ± SD of the relative number of total cells per lineage. Eosinophils were included in granulocyte counts.  

  •  P0/0 × TCR mice had all died by day 14.