Table 1

Maximal CFU-GM/5 × 102 Sca1+/lin/dim in Response to Cytokine Stimulation

CytokineGenotype
−/−+/−+/+
GM-CSF (10 ng/ml)71 ± 8.5 70 ± 11.268 ± 7.5
IL-3 (50 U/ml) 73 ± 15.6 68 ± 14.4 80 ± 14.3
GM-CSF (10 ng/ml) +
 SCF (10 ng/ml)119 ± 23.0 89 ± 15.6 96 ± 12.3
IL-3 (50 U/ml) +
 SCF (10 ng/ml)117 ± 20.8110 ± 12.7105 ± 15.6
SCF (100 ng/ml) 57 ± 16.147 ± 6.546 ± 8.3
  • The colonies enumerated from each respective genotype after stimulation with cytokines that yielded maximum colony activity. (See Fig. 2). Results are expressed as mean ± SE. All data represent analysis from four independent experiments.