Table 2

Peripheral Blood Values of LCMV-GP tg Mice after Adoptive Transfer with LCMV-GP–specific T Cells

Donor T cells*Recipient‡Infection of recipientInfiltration of isletsCBC§ of recipients (10 d after adoptive transfer)
ReticulocytesPlateletsNeutrophils
NoneNo transgeneNone215 ± 10    953 ± 1104.5 ± 0.2
NoneNo transgenevacc-LCMV-GP210 ± 281,021 ± 923.8 ± 0.1
P0/0 × TCRLCMV-GPvacc-LCMV-GP++++221 ± 151,271 ± 1454.0 ± 0.4
P0/0 × TCRNo transgenevacc-LCMV-GP229 ± 25    998 ± 844.7 ± 0.3
  • *  LCMV-GP–specific T cells were activated by infecting P0/0 × TCR mice with 2 × 106 PFU vacc-LCMV-GP intravenously. 6 d later, 5 × 106 spleen cells were transferred into syngeneic recipients (H-2b).  

  •  Recipient mice expressed LCMV-GP as a transgene under the rat insulin promoter in the β cells of the pancreatic islets (47). To keep the transferred cells activated, recipient mice were infected intravenously with 2 × 106 PFU vacc-LCMV-GP after transfer. Pancreatic islets were analyzed histologically and have been described previously (21).  

  • §  CBCs were quantified in a hemocytometer, and neutrophils were determined microscopically from blood smears. Values are ×106/ml.