Table 1

Calcium-free Media Increase Yields of Lymph Node DCs

Tissue/mediumnTotal cells (×107)Percentage of low densityPercentage of CD11c+
Lymph node, Hanks2 2.31 ± 0.452.4 ± 0.020.04 ± 0.05
Lymph node, Ca-free7 2.32 ± 0.253.0 ± 0.590.84 ± 0.11
Spleen, Hanks319.6 ± 0.98.8 ± 0.89       0.67 ± 0.06 (10.6)
Spleen, Ca-free720.0 ± 1.54.3 ± 0.78           0.95 ± 0.19 (22.0)
  • *  In spleen, the percentages of T cell area DCs are in parentheses and correspond to the percentage of DEC-205 or CD8 positive cells in the CD11c+ fraction.