Table 2

In Vitro Control of T. gondii Replication by Peritoneal Exudate Cells Harvested from Naive and 5-d Infected iNOS ko or C57BL/6 Mice

In vitro stimulusTachyzoite/cell ratioNormal resident cellsT. gondii elicited cells
C57BL/6iNOS koC57BL/6iNOS ko
cpm% killingcpm% killingcpm% killingcpm% killing
None0 43 ± 2274 ± 53294 ± 49181 ± 21
None0.23146 ± 478 2746 ± 660225 ± 741866 ± 44
None17554 ± 2028654 ± 1489226 ± 394882 ± 815
IFN-γ0 58 ± 1554 ± 20125 ± 2697 ± 9
IFN-γ0.2300 ± 3392.2 1827 ± 31731.0205 ± 9003031 ± 880
IFN-γ11180 ± 12685.1 5997 ± 89229.9188 ± 3505023 ± 8220
  • Mice (3–5 per group) were infected with 20 ME49 cysts each and 5 d later the animals were killed and peritoneal cells were isolated and pooled. The cells were next precultured in the presence or absence of IFN-γ, infected and harvested as described in Table 1. Percentage killing of tachyzoites was then calculated. The experiment shown is representative of four performed.