Table 2

Human CII Determinants Recognized by T Cells from B10.M and B10.M-DR1 Mice

T cell determinantPeptide*Peptide sequence‡T cell stimulation§
hCII (247–261)   GPLGPKGQTGEBGIA
B10.M determinanthCII (250–264)      GPKGQTGEBGIAGFK+
hCII (253–267)         GQTGEBGIAGFKGEQ
B10.M-DR1hCII (256–270)            GEBGIAGFKGEQGPK+
Dominant determinanthCII (259–273)               GIAGFKGEQGPKGEB+
hCII (262–276)                  GFKGEQGPKGEBGPA+
hCII (265–279)                     GEQGPKGEBGPAGPQ
hCII (283–297)GPAGEEGKRGARGEB
B10.M-DR1hCII (286–300)   GEEGKRGARGEBGGV+
SubdominanthCII (289–303)      GKRGARGEBGGVGPI+
determinanthCII (292–306)         GARGEBGGVGPIGPB+
hCII (295–309)            GEBGGVGPIGPBGER
  • *  Mimotope synthetic peptides. Sequence numbers are based on assigning the first amino acid of the human α1(II) chain as residue number 1.  

  •  Synthetic peptides are aligned according to sequence overlap used in the Mimotope analysis. Alignment allows for deduction of determinant core (underlined text) when more than one peptide is stimulatory for T cells. Amino acids are represented by single letter code with B denoting hydroxyproline.  

  • §  Stimulation data summarized from T cell proliferation data in Fig. 6.