Table 3

BM Cytology of Wild type, IFN-α/β R0/0, and IFN-γ R0/0 Mice Infected with LCMV-WE

GenotypeDays after infection*Megakaryocyte§Cell type (%)‡
ErythroblastMyeloblast promyelocyteMyelocyte metamyelocyte bandPolymorphMonocyteLymphocyte
Wild typeUninfected17–25   35.5 ± 1 9.5 ± 1.836.5 ± 2.8 7.7 ± 1.50.8 ± 0.3   10 ± 1.4
Wild type3 4–1115.5 ± 1.428.5 ± 1.337.5 ± 2.5 4.0 ± 1<0.514.5 ± 2.3
IFN-α/β R0/0321–2637.5 ± 1.510.5 ± 0.533.5 ± 2.612.0 ± 21.0 ± 0.4 5.5 ± 0.1
IFN-γ R0/03 8–1317.0 ± 1.828.5 ± 1.839.5 ± 3.2 3.5 ± 0.5<0.511.5 ± 0.3
  • *  Sex-matched mice (8–12 wk) of different genotypes (three mice per group) were infected with LCMV-WE (2 × 106 PFU). At the time points indicated, BM smears were stained with May-Günwald/Giemsa, chloracetate esterase, α-naphthyl butyrate esterase, and Sudan black B. Differential counts were obtained microscopically by counting 200 or more cells.  

  •  Data are means ± SD of the relative number of total cells per lineage. Eosinophils were included in granulocyte counts.  

  • § Numbers represent megakaryocytes per visual field at a magnification of ×80. They were determined semiquantitatively by counting at least four visual fields.