Table 1

Leukocytes in Epidermal Cell Suspensions from TGF-β1 Null and Control Mice

ExperimentAgeGenotypeLC (%)*DETC (%)*Leukocytes (%)*
1 8d−/−0.2ND 2.5
+/+5.5ND 6.5
210d−/−0.22.7 (3.2) 3.6
+/+5.43.8 (4.2) 8.5
318d−/− 04.9 4.8
+/−3.83.5 6.3
+/+4.54.410.5
417d−/−0.1ND 6.7
+/−6.1ND13.0
+/+6.5ND11.9
514d−/−0.1ND 6.7
+/−5.4ND10.6
+/+8.9ND13.7
617d−/− 07.6 7.6
+/+5.26.612.4
714d−/− 0ND 6.9
+/+3.0ND 6.5
Summary**
−/−0.1 ± 0.1  (n = 7)5.1 ± 2.0  (n = 3) 5.5 ± 1.8  (n = 7)
+/−5.1 ± 1.0  (n = 3)3.5  (n = 1)10.0 ± 2.8  (n = 3)
+/+5.6 ± 1.7  (n = 7)4.9 ± 1.2  (n = 3)10.0 ± 2.7  (n = 7)
  • Epidermal cell suspensions were prepared from TGF-β1 −/− mice and littermate controls and stained for CD45 and I-A antigens (LC), CD45 and TCRγδ (DETC), and CD45 and isotype control (Leukocytes), and analyzed using multicolor flow cytometry. In some experiments, DETC were also identified as CD45+Thy-1.2+ cells (% in parentheses). Nonviable cells were excluded by live gating. Data are presented as percentages of events accumulated and represent individual determinations (*) or the mean ± SEM (**) of individual determinations.