Table 1

Immunohistochemical Characterization of Cellular Infiltrates in Ears Following Topical Challenge

MiceCell typeT cell adoptive transfer*S.C. immunization*Nonspecific irritant*
SensitizedNonsensitizedSensitizedNonsensitizedSensitizedNonsensitized
+/+Mac/Gran527 ± 124§33 ± 26553 ± 12019 ± 253,535 ± 950   100 ± 25
−/−Mac/Gran572 ± 14215 ± 24540 ± 14318 ± 213,375 ± 117236 ± 120
+/+Gran150 ± 9518 ± 21252 ± 15318 ± 253,467 ± 96   335 ± 12
−/−Gran276 ± 14120 ± 20265 ± 10117 ± 193,507 ± 95   184 ± 57
+/+T cells107 ± 597 ± 6121 ± 4325 ± 250
−/−T cells 91 ± 37    12 ± 4119 ± 4129 ± 270
  • *T cell Adoptive Transfer: purified +/+ PLN T cells from DNFB-sensitized or control mice were injected intravenously into +/+ and −/− mice. Responses were elicited with DNFB 12 h after cell injection. S.C. Immunization: DNBS-treated or control −/− spleen cells were injected subcutaneously on the dorsum +/+ and −/− mice. Responses were elicited with DNFB 5 d after cell injection. Nonspecific Irritant: 10 μl of 0.8% croton oil was painted onto the pinna of +/+ and −/− mice. Ears were harvested 24 h following DNFB challenge or 16 h following application of croton oil, embedded, and stained.  

  • Mac/Gran: macrophages plus granulocytes, anti-Mac-1. Gran: granulocytes only, anti–GR-1. T cells, anti-Th1.2.  

  • §  Cells/mm2.  

  •  Not done.