Table II.

Mutations within CD81 binding domains of viral variants

Viral variantsCD81 BDI amino acid positionsCD81 BDII amino acid positionsCD81 BDIII amino acid positions
412414416420474475476477478479480481492521522524528531533537538
P01VLaQVTWYAESRSSDQRSVTEKLL
P01VAa
P01VBb
P01VCbCK
P01VIbL
P01VKbPLFAE
P01VJcIPRL
P02VIaQITWYAEPHDLGQRSANEELI
P02VJaQDR
P02VHaQDR
P02VAaVD
P02VDaVDR
P02VBbLAD
P02VFcSRSDR
P02VGcVDRT
P03VCaKVTWYAKPNSSDRRFASEELL
P03VAaQET
P03VBbE
P04VEaQITWYAEPRSLDQRFVNEELL
P04VDa
P04VCb
P04VAbNK
P04VBb
P05VEaQVTWYTEPEALDQRFVSEELL
P05VFa
P05VDa
P05VAc
P05VBc
P05VCc
P06VHaQITWHVVPHGLDRRFATEEFI
P06VIaR
P06VDaQ
P06VGbAR
P06VFbYAESRSVNLL
P06VAcYAERS
P06VBcYAERSVNLL
P06VCc
  • BD, binding domain; RLU, relative light unit. For each patient, amino acid changes in the CD81 BDI, BDII, and BDIII (Owsianka et al., 2006) relative to the most prevalent variant showing efficient viral entry are indicated. Amino acid positions where at least one mutation was observed in the six patients are shown; conserved amino acid positions are not depicted.

  • a Variants showing high infectivity (RLU ≥ 5 log10).

  • b Variants showing low infectivity (5 log 10 ≥ RLU ≤ 3.5 log10).

  • c Noninfectious variants (RLU ≤ 3 log10).