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The Journal of Experimental Medicine 194 12 The endocytic adaptor intersectin 2 mediates Wiskott Aldrich Syndrome protein (WASp) translocation from the actin cytoskeleton to endocytic vesicles. Cos 7 cells were transfected with green fluorescent protein (GFP)-WASp (left), GFP-intersectin 2 (middle), or GFP-intersectin 2 and DsRED-WASp (right). Rhodamine-phalloidin staining of these cells for actin visualization reveals WASp to colocalize with actin in perinuclear cytoskeletal bundles (yellow staining, left panel) while intersectin 2 localizes independently of actin within small endocytic vesicles (green staining, middle panel). When coexpressed with intersectin 2, WASp translocates within the cell to colocalize with intersectin 2 in endocytic vesicles (yellow staining, right panel). These data reveal the capacity of intersectin 2 to redirect WASp into endocytic vesicles and to thereby couple the endocytic pathway to the actin nucleation machinery within the cell. See related article by McGavin et al., pp. 1777-1787
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