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Video 3
Real-time imaging of prolonged interaction of B cells with FDCs (example 1, 50 min; example 2, 45 min). Two time-lapse image sequences of 20-┬Ám z projection from inguinal LN explants. These are examples of CFSE-labeled MD4 B cells (green; circled in the first few frames) interacting with FDCs for long periods. The minimum observable contact times of these cells are 40 min in example 1, and 22 and 15 min in example 2. Noncognate CFP tg B cells are observed in cyan some of which are also weakly detected in the green channel. Elapsed time is shown as hours:minutes:seconds. Data are from two different inguinal LNs representative of three experiments.