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Figure S4.  IL-12a (p35) expression was compared in WT, Fcγ−/−, and Fcγ−/−.IL-12a+/− mice. Spleen was taken from each mouse, and splenic DCs were purified as described in Materials and methods. 1 µg of total RNA was converted to cDNA and real-time PCR was performed. Polr2a was amplified as a housekeeping gene, and relative fold induction was calculated as described in the Fig. S4 legend. Each dot represents an individual mouse. *, P ≤ 0.05. Horizontal bars show the mean of five individual mice.