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Figure S2. Increased expression of activation markers on Fcγ−/− BMDC. 6–8-wk old WT and Fcγ−/− mice were killed and femurs were prepared. BM cells were obtained by flushing the femurs with PBS and non-DC precursor cells were eliminated as described in Materials and methods. DC precursor cells were counted and 106/ml cells were cultured in complete RPMI 1640 supplemented with GM-CSF for 6 d. On day 6, nonadherent immature DCs were collected and further cultured with medium alone with or 1 µg/ml LPS. After 2 d, cells were harvested and stained for CD11c and the activation markers I-Ad and CD86. The percent of surface marker–positive cells and their MFI was analyzed on an LSRII. n = 5; *, P ≤ 0.05; **, P ≤ 0.01. Error bars show mean ± SD.