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Video 3
Induction of repetitive Ca2+ increases in one T cell during its migration within the slice. The lymphocyte was loaded with fura-2 AM and overlaid onto a LN slice 1 h before the recording. Red dots mark the position of the T cell at successive 30-s intervals. The intracellular Ca2+ level is displayed in colors ranging from blue (50-100 nM) to yellow (300-400 nM).