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Video 2
T cells are highly motile within a LN slice. A LN slice was stained with ER-TR7 antibody (red), and fluorescently labeled T cells (green) were added. 1 h later, images were recorded every 30 s for 20 min. The animation represents a 40-┬Ám z-projection. The ER-TR7 staining clearly visualizes the capsule as well as a region (top), which is most likely the medulla.