[HELP with High Resolution Image Viewing]

[View Larger Version of this Image]

Figure S4. >Localization of CCL21 and CXCL12 in LN slices. (A, left) Control staining. Anti-CCL21 antibody was preincubated with the recombinant chemokine. (right) CCL21 staining with anti-CCL21 antibody. (B) CCL21 detection in the T zone versus ER-TR7 localization. Bar, 50 µm. (C, left) CXCL12 detection with a biotinylated anti-CXCL12 antibody. (right) As a control, the LN slice was preincubated with nonbiotinylated anti-CXCL12. (D) CXCL12 detection in the T zone versus ER-TR7 localization. Bar, 50 µm. In A and B, fluorescence intensity is coded in pseudocolors ranging from dark blue (weak signal) to red-white (strong signal).