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Figure S5. Local radiation of tumors increases Ag cross-presentation of stromal cells in a dose-dependent manner. 2 x 106 MC57-SIY-Lo cells were injected into each flank of C57BL/6 Rag1-/- mice. After 14 d, MC57-SIY-Lo tumors were locally irradiated with doses ranging from 1 to 10 Gy. 2 d later, tumors were excised, dispersed as single-cell suspensions, and stained with APC-conjugated anti-CD11b antibody and the SIY-Kb-specific m67 TCR tetramers. MFI, mean fluorescence intensity.