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Figure S3. Endogenous TGF-β enhances IL-22 expression in the context of proinflammatory cytokines. Naive DO11 cells were activated with 1 µg/ml OVAp, irradiated splenocytes, and various cytokines and antibody as indicated. Anti-TGF-β (1D11; R&D Systems) was used at 10 µg/ml. All other cytokines were used at concentrations indicated in Materials and methods. Intracellular cytokine staining was performed on days 2 and 5.