[HELP with High Resolution Image Viewing]

[View Larger Version of this Image]

Figure S1. Oral streptomycin administration during intestinal bacterial infection does not facilitate streptomycin leakage into the bloodstream. Mice were either infected with 5 x 108 CFU of Y. pseudotuberculosis or mock-infected with PBS, followed by oral gavage with 1 mg of streptomycin at 5, 12, and 24 h after infection. Serum streptomycin levels were determined at 24 h after infection. Controls included serum from uninfected mice (normal mouse serum), serum from mice injected intravenously with 200 µg of streptomycin, and normal mouse serum spiked in vitro with streptomycin to give 100 µg/mL final concentration. Results shown are average serum streptomycin levels from two to four samples (each sample containing serum pooled from four to five mice); error bars indicate standard deviation.