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Figure S1. Regulation of tumor B7-H4 expression. Established ovarian tumor cells were cultured for 72 h in the indicated conditions. Surface B7-H4 expression was detected by FACS (A and B) and RT-PCR (C). Tumor ascites, recombinant IL-6, IL-10, IL-4, and GM-CSF did not affect B7-H4 protein (A and B) and mRNA (C) expression in ovarian tumor cells. Results were expressed as the mean ± SEM of three individual tumor cell lines (OC8, OC21, and OC38).