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Video 1
Real-time imaging of B cell migration in the FDC network (90 min). Time-lapse image sequence of 20-┬Ám z projection from an inguinal lLN explant showing that CFSE-labeled MD4 B cells (green) and noncognate CFP-tg B cells (cyan) are migrating through the FDC network visualized with HEL-PE (red). Several MD4 B cells carry HEL-PE at their uropod and are tracked with white dots. Capsule-associated collagen fibers appear blue by second harmonic emission. Elapsed time is shown as hours:minutes:seconds. Data are representative of three experiments.