High Levels of a Major Histocompatibility Complex II–Self Peptide Complex on Dendritic Cells from the T Cell Areas of Lymph Nodes

Y-Ae antibody was provided by C. Janeway (Yale University, New Haven, CT) and used as a purified Ig. The Y-Ae mAb recognizes a complex formed between I-A^b and an I-Eα peptide (6). Other mAbs were purchased from PharMingen (San Diego, CA) or American Type Culture Collection (Rockville, MD).

C57BL6 × DBA/2 and BALB/c × C57BL/6 F1 mice (6–10 wk, both sexes) express MHC–peptide complexes recognized by the Y-Ae mAb, while C57BL/6 (I-A^b only) and BALB/c (I-Eα peptide only) do not.

Lymph nodes and spleens were dissociated with collagenase (collagenase D; Boehringer Mannheim Biochemicals, Indianapolis, IN) at 100 U/ml during the teasing apart of the tissue and then at 400 U/ml for 30 min at 37°C. The suspension was then immediately diluted 1:10 in Ca²⁺-free Hanks solution, centrifuged, and suspended in dense BSA prepared as described (7). A low density cell fraction, enriched in DCs, was harvested after centrifugation at 900 g for 30 min at 4°C. The cells were washed in Ca²⁺-free Hanks solution. DCs were also prepared from bone marrow precursors and epidermis as described (8, 9).

10-μm cryostat sections were applied to multiwell slides (No. 111006; Carlson Scientific, Inc., Peotone, IL), air dried, and fixed in acetone for 10 min at room temperature. Biotinylated mAbs (CD8 for T cells; DEC-205, 2A1, MIDC-8, and CD11c for DCs; and Y-Ae for MHC peptide complexes) were applied at 1 μg/ml for 1 h, the sections were washed in PBS, and alkaline phosphatase–coupled, avidin biotin complexes (AK-5000 alkaline phosphatase standard kit; Vector Labs Inc., Burlingame, CA) were applied for 30 min. The sections were washed and the blue alkaline phosphatase reaction product developed with a substrate kit (SK-5300; Vector Labs). Rat mAb was then added as a culture supernatant for 1 h. After washing, horseradish peroxidase–conjugated F(ab)′₂ anti–rat Ig (No. 212-036-082; Jackson ImmunoResearch Labs Inc., West Grove, PA) was reconstituted as recommended for extended storage, diluted 1:200, and applied for 1 h. DAB (Stable DAB No. 750118; Research Genetics, Huntsville, AL) was then used to develop a brown reaction product. The slides were mounted in PBS glycerol, followed by a peroxidase avidin complex and brown diaminobenzidine reaction product.

Biotin-modified mAbs were prepared for CD11c, Y-Ae, and MHC II (clone M5/114) and identified with PE-streptavidin. Rat mAbs were used as hybridoma culture supernatants and identified with FITC-mouse anti–rat Ig (Boehringer Mannheim). We purchased FITC-modified mAb to CD40, CD4, CD8, CD54, and CD86 from PharMingen. In most experiments, cells were fixed and permeabilized, since many of the antigens were primarily intracellular in DCs (DEC-205, MIDC-8, and 2A1). Cells were fixed in 4% paraformaldehyde (Fisher Scientific Co., Pittsburgh, PA) for 30 min, washed twice with PBS, permeabilized with 0.5% saponin (Sigma Chemical Co., St. Louis, MO) in PBS with 1% FCS at 4°C for 10 min, and then washed twice with staining medium.

FACS^® Purification of Lymph Node DCs and B Cells. Starting with a low density fraction from BSA columns (see above; 3–4% of lymph node cells) CD11c⁺ B220⁻ DCs were sorted from CD11c⁻ B220⁺ B cells on a FACStar^PLUS instrument and with FITC-CD11c and PE-B220 mAbs from Endogen, Inc. (Boston, MA) and PharMingen, respectively. The sorted fractions were confirmed to be >98% pure.

T-T hybrids, specific for a Y–Ae complex, were provided by L. Ignatowitz (University of Colorado, Denver, CO; reference 10). The response to APCs was assessed by IL-2 release or apoptosis 18 h later. IL-2 release was measured in a bioassay on conA activated T blasts and the CTLL line. Apoptosis was measured as a decrease in ³H-TdR uptake, and reduced or hypodiploid DNA staining with propidium iodide, 50 μg/ml in 0.1% sodium citrate and 0.1% Triton X-100. The APCs were FACS^® purified DCs or B cells added in graded doses to 10⁵ T-T hybridoma cells.

The Y-Ae monoclonal recognizes a complex of an I-Eα peptide presented on I-A^b MHC class II molecules (6). Tissue sections of C57BL6 × DBA/2 (I-A^b x I-Eα) lymph nodes which were stained with Y-Ae showed stronger reactivity in T cell areas relative to B cell follicles (Fig. 1). The strong Y-Ae staining in T areas was likely on DCs, since mouse T cells do not express MHC class II and since T cell areas have relatively few macrophages and B cells (11, 12). Prior work had shown strong staining with Y-Ae in thymic medulla (13), but this site has numerous medullary epithelial cells in addition to DCs (14).

To identify and isolate the cells which expressed high levels of MHC II–self peptide complexes from lymph nodes, we first stained sections with a panel of monoclonals which react with isolated mature DCs but not with T cells and thymocytes. T cell areas contained many profiles which labeled for (a) a panel of mAbs which react with antigens within intracellular granules of mature DCs and are termed 2A1 (8), M342 (15), and MIDC-8 (16); (b) the CD11c integrin which is abundant on DCs (17, 18), and (c) the DEC-205 multilectin receptor expressed by DCs (19–21). The expression of each antigen was weaker (2A1) or not detected (CD11c, DEC-205, M342, and MIDC-8) on B cells. Antigen-presenting (MHC II, invariant chain) and costimulatory (CD54 and CD86) molecules also were prominently displayed on dendritic profiles in the T cell areas (not shown, but for reviews see references 11 and 22).

Fig. 2 illustrates the distribution of the 2A1 staining. This monoclonal stains granules within isolated mature DCs as described (8), but the molecule expressing the 2A1 antigen has yet to be identified. In sections, strong staining is seen on many profiles in the T cell areas, with weaker staining in the B cell areas. However, 2A1 staining is not seen on most DCs which are isolated by earlier methods from spleen and lymph node. Two other mAbs, M342 (15) and MIDC-8 (16), also do not stain freshly isolated less mature DCs but only stain after the cells have been cultured overnight.

By emphasizing the use of Ca²⁺-free media (23), we were able to release and enrich (on BSA columns) cells with all the above markers of T cell area DCs from lymph nodes. Standard dissociation procedures with collagenase yielded very few cells that stained for DEC-205, CD11c, or the 2A1, M342, and MIDC-8 granule antigens which are found on T cell area DCs (Fig. 2). However Ca²⁺-free media increased the yield of CD11c⁺ cells 25-fold from lymph node (Table 1). The large cells had all the markers of T cell area DCs, including large cell size, irregular shape, abundant MHC II, DEC-205, and granule antigens (Fig. 3). Most other low density cells were smaller, CD11c⁻ B220⁺ B cells (Fig. 3, right). Few macrophages were present, by cytology or staining for SER-4 (24) and F4/80 (12) antigens.

The use of Ca²⁺-free media followed by flotation on dense BSA allowed the isolation of cells which were B220⁻ (not B cells) but had very high levels of Y-Ae MHC–peptide complex, higher than the B220⁺ B cells (Fig. 4,A, compare populations with black and white arrows). The Y-Ae⁺ cells were only found in mice which expressed both I-A^b (C57BL/6) and the I-Eα peptide (BALB/c)(Fig. 4,A). If the low density cells from BALB/c × C57BL/6 F1 mice were double labeled for B220 and either CD11c integrin or Y-Ae, it was clear that CD11c high and Y-Ae high populations (Fig. 4 B, black arrow), were B220⁻. The B220⁺ cells had little or no CD11c but intermediate levels of Y-Ae. By sorting cells into CD11c⁺ B220⁻ and CD11c⁻ B220⁺ fractions, we were able to obtain 98% pure populations of DCs and B cells, respectively.

The CD11c⁺ cells were then labeled with a panel of mAbs to ensure that they carried the markers of T cell area DCs and to look for other cell-specific and costimulatory molecules. CD11c⁺ lymph node cells carried the same markers as DCs in sections of T cell areas (Fig. 5, top row, black arrows). DEC-205, 2A1, and MIDC-8 (latter not shown) were all abundant on most CD11c⁺ cells, as was CD86, CD54, CD40, and MHC I (latter not shown). The phagocyte markers, SER-4/sialoadhesin and RB6/GR-1, were not expressed, and the T cell markers, CD8 and CD4, were weak on most profiles (Fig. 5). In mesenteric nodes, or in peripheral lymph nodes stimulated with incomplete Freund's adjuvant, most of the cells with T cell markers had high levels of CD11c, whereas with unstimulated peripheral lymph node (axillary, inguinal, and cervical), many of the DCs had intermediate levels of CD11c (not shown).

In the spleen (Fig. 5), two populations of CD11c⁺ cells were evident. About 50% expressed high levels of CD8 and DEC-205, but only a fraction had high levels of 2A1. The CD11c-rich cells had the highest levels of the CD86, CD54, and CD40 accessory molecules (Fig. 5). We conclude that the majority of CD11c⁺ cells that were released under Ca²⁺-free conditions from lymph node, and to a lesser extent spleen, carried the DC markers which were found in sections through the T cell areas.

Semiquantitative FACS^® data on the markers of cells expressing the I-A^b/I-Eα, MHC II-peptide complexes were then obtained. The cells with the highest labeling for Y-Ae in lymph node (Fig. 5, black arrows) carried all the markers of T cell area DCs (CD11c, high MIDC-8, DEC-205, and 2A1), as well as the highest levels of MHC I, CD40, CD54, CD86, and heat stable antigen. This was especially impressive in spleen where the cells with the most abundant Y-Ae were exclusively those with the DEC-205 and 2A1 DC markers (Fig. 5, black arrows).

To identify cells with strong staining for DEC-205, granule antigens like 2A1 and MIDC-8, and the lysosomal markers CD107a (LAMP-1) and CD68, it was necessary to permeabilize the cells with saponin before staining. The different staining results with fixed and fixed permeabilized cells are illustrated in Fig. 6 (compare F with FP).

To rule out acquisition of I-Eα peptide from B cells, we stained DC suspensions which lacked B cells, i.e., DCs from epidermis (9) and from bone marrow–progenitors (8). Both epidermal (Fig. 7,A) and bone marrow–derived (Fig. 7 B) DCs expressed high levels of Y-Ae and the CD86 costimulator. Again only the cells from the appropriate mouse strain (C57BL/6 × DBA/2 F1 vs. C57BL/6) expressed the Y-Ae epitope.

FACS^®-purified, CD11c⁺ B220⁻ DCs and CD11c⁻ B220⁺ B cells were used to present the Y-Ae epitope to two T-T hybrids which recognize I-A^b/I-Eα peptide complexes (10). The DCs were >30-fold more potent in inducing IL-2 release and inducing the hybridomas to stop synthesizing DNA (Fig. 8,A), an indication of apoptosis (25). To verify that apoptosis had taken place, the cells were stained with propidium iodide and shown to have high levels of hypodiploid profiles 24 h after stimulation with DCs from the appropriate strain of mice (Fig. 8 B, compare B6D2 with B6 DCs).

An obstacle to the study of antigen presentation in vivo is the difficulty in isolating DCs from lymph nodes, the major organs in which immune responses are generated. This obstacle is paradoxical since sections of T cell areas contain many cells with features of DCs (Fig. 1 and 2; for reviews see references 3–5). In contrast, spleen yields many DCs, yet T cell areas represent a much smaller proportion of spleen than lymph node. Many of the DCs which are released from spleen emanate from the periphery of the white pulp (17, 18) and must be cultured to develop the properties of T cell area DCs, such as high expression of MIDC-8, 2A1, and M342 positive granules, DEC-205, and MHC II and CD86 (15, 22). A subset of spleen DCs which carries CD8 and has a regulatory role, dampening T cell responses, was identified using Ca²⁺-free media (23, 26, 27). By emphasizing Ca²⁺-free media, lymph node cells which have all the properties of T cell area DCs can be isolated, although CD8 expression is weak.

Functional assays with cells from different tissues have shown that DCs are the main cell type which carries foreign (28–31) and self (32) antigens. However, strong APC function may reflect either high levels of MHC–peptide complexes (“signal one”) and/or an abundance of T cell adhesion and costimulatory molecules (“signal two”), e.g., ICAM-1/CD54 and B7-2/CD86. Our data indicate that both signal one and two are abundant on T cell area DCs. The Y-Ae monoclonal directly shows that peripheral DCs are presenting MHC II–self peptide complexes in high amounts, and that these DCs can induce cell death in T-T hybridomas which are specific for the MHC II–self peptide complex. Death of T cell hybridomas occurs when DCs present foreign antigens to T-T hybrids as well (our unpublished observation).

Kurts et al. (33) recently reported functional data that an MHC I–self peptide complex from pancreatic islet β cells is expressed on bone marrow–derived APCs in the draining lymph node. They have since shown that these APCs mediate peripheral tolerance (34). DCs have long been considered as adjuvants for responses to foreign antigens, but it is possible that some T cell area DCs can have a regulatory or tolerizing role, perhaps by the expression of fas-ligand, as suggested by the data from Suss and Shortman (26). The reason to consider a role for DCs in peripheral tolerance relates to two well studied systems in vivo, presentation of H-Y antigens and superantigens. These ligands elicit strong immune responses when presented on DCs which have been allowed to mature in vitro (35, 36), yet the same antigens can be tolerogenic in vivo (37–39). Therefore DCs should be assessed in the regulation of autoreactivity and not just immunogenicity to foreign antigens.

This work was supported by grants from the Japanese Ministry of Education, Science, and Culture (08282104 and 08044271), the Yamanouchi Fund, the Juvenile Diabetes Foundation, the Human Science Frontiers Program, and National Institutes of Health grants AI-13013 and DK-39672.

DC

dendritic cell