Skip to main content

Main menu

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA

User menu

  • Log in

Search

  • Advanced search
JEM
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA
  • Log in
JEM

Advanced Search

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions
Article

Expression of the zinc finger transcription factor zDC (Zbtb46, Btbd4) defines the classical dendritic cell lineage

Matthew M. Meredith, Kang Liu, Guillaume Darrasse-Jeze, Alice O. Kamphorst, Heidi A. Schreiber, Pierre Guermonprez, Juliana Idoyaga, Cheolho Cheong, Kai-Hui Yao, Rachel E. Niec, Michel C. Nussenzweig
Matthew M. Meredith
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kang Liu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guillaume Darrasse-Jeze
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alice O. Kamphorst
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Heidi A. Schreiber
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pierre Guermonprez
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juliana Idoyaga
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cheolho Cheong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kai-Hui Yao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel E. Niec
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michel C. Nussenzweig
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1084/jem.20112675 | Published May 21, 2012
  • Article
  • Figures & Data
  • Info
  • Metrics
  • Preview PDF
Loading

Abstract

Classical dendritic cells (cDCs), monocytes, and plasmacytoid DCs (pDCs) arise from a common bone marrow precursor (macrophage and DC progenitors [MDPs]) and express many of the same surface markers, including CD11c. We describe a previously uncharacterized zinc finger transcription factor, zDC (Zbtb46, Btbd4), which is specifically expressed by cDCs and committed cDC precursors but not by monocytes, pDCs, or other immune cell populations. We inserted diphtheria toxin (DT) receptor (DTR) cDNA into the 3′ UTR of the zDC locus to serve as an indicator of zDC expression and as a means to specifically deplete cDCs. Mice bearing this knockin express DTR in cDCs but not other immune cell populations, and DT injection into zDC-DTR bone marrow chimeras results in cDC depletion. In contrast to previously characterized CD11c-DTR mice, non-cDCs, including pDCs, monocytes, macrophages, and NK cells, were spared after DT injection in zDC-DTR mice. We compared immune responses to Toxoplasma gondii and MO4 melanoma in DT-treated zDC- and CD11c-DTR mice and found that immunity was only partially impaired in zDC-DTR mice. Our results indicate that CD11c-expressing non-cDCs make significant contributions to initiating immunity to parasites and tumors.

  • Submitted: 16 December 2011
  • Accepted: 2 May 2012

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

View Full Text
© 2012 Meredith et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Next article
Back to top
Download PDF
Citation Tools
Expression of the zinc finger transcription factor zDC (Zbtb46, Btbd4) defines the classical dendritic cell lineage
Matthew M. Meredith, Kang Liu, Guillaume Darrasse-Jeze, Alice O. Kamphorst, Heidi A. Schreiber, Pierre Guermonprez, Juliana Idoyaga, Cheolho Cheong, Kai-Hui Yao, Rachel E. Niec, Michel C. Nussenzweig
Journal of Experimental Medicine May 2012, jem.20112675; DOI: 10.1084/jem.20112675

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts
Sign In to Email Alerts with your Email Address

Email logo Twitter logo Facebook logo Mendeley logo Reddit logo CiteULike logo LinkedIn logo

The Journal of Experimental Medicine: 215 (4)

Current Issue

April 2, 2018
Volume 215, No. 4

  • Table of Contents
  • All Issues

Jump To

  • Article
    • Abstract
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info
  • Metrics
  • Preview PDF
 

ARTICLES

  • Current Issue
  • Newest Articles
  • Archive
  • Alerts
  • RSS feeds

FOR AUTHORS

  • Submit a Manuscript
  • Instructions for Authors

ABOUT

  • About JEM
  • Editors & Staff
  • Policies & Permissions
  • Advertise
  • Contact Us
  • Feedback
  • Newsroom

CONNECT WITH JEM

  • Email
  • Facebook
  • Twitter
  • RSS
  • Instagram

Online ISSN: 1540-9538
Print ISSN: 0022-1007

Copyright © 2018 JEM by Rockefeller University Press