July 2012 | Volume 209, No. 7
Brief Definitive Report
- Runx1–Cbfβ facilitates early B lymphocyte development by regulating expression of Ebf1
Mb1-Cre–mediated deletion of Runx1 impairs B lineage development and causes accumulation of repressive histone marks on the Ebf1 proximal promoter; ectopic expression of Ebf1 but not Pax5 restores B cell differentiation.
- Bicarbonate and functional CFTR channel are required for proper mucin secretion and link cystic fibrosis with its mucus phenotype
Ileal mucus in CftrΔ508 mice is more adherent, denser, and less penetrable than that of WT mice, but addition of bicarbonate normalizes the properties of CftrΔ508 mucus.
- Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening
A human antibody facilitates opsonophagocytic killing, inhibits attachment of Pseudomonas aeruginosa, and exerts protective effects in several animal models of P. aeruginosa infection.
- SCFβ-TRCP suppresses angiogenesis and thyroid cancer cell migration by promoting ubiquitination and destruction of VEGF receptor 2
The E3 ubiquitin ligase β-TRCP, acting in concert with casein kinase I, drives ubiquitination and degradation of VEGFR2, and renders human papillary thyroid cancer cells resistant to the VEGFR2 inhibitor sorafenib.
- Identification of a genetic locus controlling bacteria-driven colitis and associated cancer through effects on innate inflammation
A genetic locus acting in hematopoietic cells regulates cytokine expression and granulocyte recruitment to confer susceptibility in a Helicobacter hepaticus–induced model of innate colitis and associated cancer.
- Janus-like opposing roles of CD47 in autoimmune brain inflammation in humans and mice
CD47 exerts different effects on disease in distinct cell types and locations and during different stages of experimental autoimmune encephalomyelitis.
- Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation
Monocytes exposed to serum from SLE patients promote B cell differentiation to IgG and IgA plasmablasts dependent on BAFF and IL-10 or APRIL, respectively.
- UNG shapes the specificity of AID-induced somatic hypermutation
UNG activity repairs activation-induced deaminase-generated U:G mismatches via error-prone or error-free repair, depending on the sequence context of the deaminated cytosine.