The class II transactivator (CIITA) and B cell octamer-binding protein 1/octamer-binding factor 1/Oct coactivator from B cells (Bob1/OBF-1/OCA-B) represent two B cell-specific transcriptional coactivators. CIITA and Bob1 interact with proteins that bind to conserved upstream sequences in promoters of class II major histocompatibility genes and octamer-binding transcription factors Oct-1 and Oct-2, respectively. Both CIITA and Bob1 increase the expression from the DRA promoter, which is a prototypic class II promoter. Moreover, in the presence of CIITA, interactions between class II promoters and Bob1 are independent of the octamer-binding site. Using in vivo and in vitro binding assays, we confirm that Bob1 binds to CIITA. Thus, CIITA not only activates the expression of class II genes but recruits another B cell-specific coactivator to increase transcriptional activity of class II promoters in B cells.
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June 01 1996
Binding and cooperative interactions between two B cell-specific transcriptional coactivators.
J D Fontes,
J D Fontes
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
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N Jabrane-Ferrat,
N Jabrane-Ferrat
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
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C R Toth,
C R Toth
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
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B M Peterlin
B M Peterlin
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
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J D Fontes
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
N Jabrane-Ferrat
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
C R Toth
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
B M Peterlin
Howard Hughes Medical Institute, Departments of Medicine, University of California, at San Francisco 94143-0724, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 183 (6): 2517–2521.
Citation
J D Fontes, N Jabrane-Ferrat, C R Toth, B M Peterlin; Binding and cooperative interactions between two B cell-specific transcriptional coactivators.. J Exp Med 1 June 1996; 183 (6): 2517–2521. doi: https://doi.org/10.1084/jem.183.6.2517
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