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jem Home » 1995 Archive » 1 November » 182 (5): 1603
Article

CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73.

L Airas, J Hellman, M Salmi, P Bono, T Puurunen, D J Smith, S Jalkanen
L Airas
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J Hellman
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M Salmi
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P Bono
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T Puurunen
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D J Smith
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S Jalkanen
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DOI: 10.1084/jem.182.5.1603 | Published November 1, 1995
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Abstract

We have recently described a monoclonal antibody (mAb) 4G4 recognizing a 70-kD molecule constitutively expressed on human endothelial cells and on subpopulations of lymphocytes. We showed that this molecule, which we named lymphocyte-vascular adhesion protein 2 (L-VAP-2), mediates lymphocyte adhesion to cultured endothelial cells. Protein sequencing of tryptic peptides from immunoaffinity-purified L-VAP-2 revealed sequence identity between L-VAP-2 and CD73 (ecto-5'-nucleotidase, E.C.3.1.3.5), and COS cells transfected with a CD73 cDNA were positively stained with the mAb 4G4, which recognizes L-VAP-2. mAb 4G4 was also able to partially inhibit the ecto-5'-nucleotidase activity of peripheral blood lymphocytes. Moreover, cross-precipitation studies performed with mAb 4G4 and a CD73 workshop mAb 1E9 showed that these two antibodies recognize the same molecule. Since the tissue distribution and biochemical characteristics of the two molecules are also similar, we conclude that L-VAP-2 and CD73 are the same glycoprotein. Adhesion experiments showed significantly increased binding of freshly isolated lymphocytes to COS cells transfected with a CD73 cDNA, as compared to mock-transfected COS cells, and binding of lymphocytes to CD73-expressing COS cells was inhibited by the presence of mAb 4G4 in the adhesion assay. CD73 is a glycosyl phosphatidylinositol-linked molecule previously shown to have a cosignalling role in T lymphocyte proliferation. Our data suggest that it also has a function in mediating lymphocyte adhesion to the endothelium.

© 1995 Rockefeller University Press
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CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73.
L Airas, J Hellman, M Salmi, P Bono, T Puurunen, D J Smith, S Jalkanen
Journal of Experimental Medicine Nov 1995, 182 (5) 1603-1608; DOI: 10.1084/jem.182.5.1603

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The Journal of Experimental Medicine: 215 (4)

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April 2, 2018
Volume 215, No. 4

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