We previously demonstrated selective enrichment of major histocompatibility complex (MHC) class II-specific autoreactive T cells in a subset of mouse CD4+ thymocytes. Here we show that a significant fraction of these autoreactive cells in the normal adult thymus expresses NK1.1 and high levels of Ly-6C and also exhibits flexibility in MHC restriction. In normal mice, this NK1.1+Ly-6Chi subfraction accounts for 10-50% of the CD4+ autoreactive subset and is enriched for MHC class II-restricted autoreactive cells as determined by mixed leukocyte reaction frequency analysis, similar to NK1.1-Ly-6C-CD4+ autoreactive cells. In contrast, in the thymus of class II-deficient littermate mice, NK1.1+Ly-6Chi cells account for most of the mature heat stable antigen (HSA)-CD4+ fraction and exhibit MHC-restricted non-class II autoreactivity. Thus, NK1.1+Ly-6ChiCD4+ T cells show flexibility in MHC class restriction, but their autoreactivity remains MHC dependent.
Article|
December 01 1994
Altered major histocompatibility complex restriction in the NK1.1+Ly-6Chi autoreactive CD4+ T cell subset from class II-deficient mice.
I Kariv,
I Kariv
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Search for other works by this author on:
R R Hardy,
R R Hardy
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Search for other works by this author on:
K Hayakawa
K Hayakawa
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Search for other works by this author on:
I Kariv
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
R R Hardy
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
K Hayakawa
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1994) 180 (6): 2419–2424.
Citation
I Kariv, R R Hardy, K Hayakawa; Altered major histocompatibility complex restriction in the NK1.1+Ly-6Chi autoreactive CD4+ T cell subset from class II-deficient mice.. J Exp Med 1 December 1994; 180 (6): 2419–2424. doi: https://doi.org/10.1084/jem.180.6.2419
Download citation file: