Pertussis toxin binds target cells through the carbohydrate recognition properties of two subunits, S2 and S3, which share amino acid sequence similarity with the lectin domains of the eukaryotic selectin family. Selectins appear on inflamed endothelial cells and promote rolling of leukocytes by reversibly binding carbohydrates. S2, S3, and synthetic peptides representing their carbohydrate recognition domains competitively inhibited adherence of neutrophils to selectin-coated surfaces and to endothelial cells in vitro. These proteins and peptides also rapidly upregulated the function of the leukocyte integrin CD11b/CD18. These findings implicate mimicry of eukaryotic selectins by prokaryotic adhesive ligands and link the mechanisms underlying leukocyte trafficking to microbial pathogenesis.
Article|
September 01 1993
Prokaryotic peptides that block leukocyte adherence to selectins.
E Rozdzinski,
E Rozdzinski
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
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W N Burnette,
W N Burnette
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
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T Jones,
T Jones
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
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V Mar,
V Mar
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
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E Tuomanen
E Tuomanen
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
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E Rozdzinski
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
W N Burnette
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
T Jones
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
V Mar
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
E Tuomanen
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1993) 178 (3): 917–924.
Citation
E Rozdzinski, W N Burnette, T Jones, V Mar, E Tuomanen; Prokaryotic peptides that block leukocyte adherence to selectins.. J Exp Med 1 September 1993; 178 (3): 917–924. doi: https://doi.org/10.1084/jem.178.3.917
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