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jem Home » 1993 Archive » 1 May » 177 (5): 1451
Article

The selective ablation of interleukin 2-producing cells isolated from transgenic mice.

L E Minasi, Y Kamogawa, S Carding, K Bottomly, R A Flavell
L E Minasi
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Y Kamogawa
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S Carding
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K Bottomly
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R A Flavell
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DOI: 10.1084/jem.177.5.1451 | Published May 1, 1993
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Abstract

To better understand the requirement for interleukin 2 (IL-2) in specific immune responses, we have established the use of cell ablation to selectively eliminate T cells that produce IL-2. To accomplish this we have generated transgenic mice that express the herpes simplex virus 1-thymidine kinase (HSV-TK) gene under the transcriptional control of the murine IL-2 promoter that renders IL-2-producing cells sensitive to the cytotoxic effects of the antiviral drug ganciclovir (GANC). HSV-TK activity was specifically expressed in activated T cells from transgenic mice. When CD4 T cells from transgenic mice were stimulated with the superantigen staphylococcal enterotoxin A (SEA) in the presence of GANC, proliferation and IL-2 production were almost completely inhibited and the activated CD4+V beta 3+ T cell population, eliminated. Proliferation was not restored by adding IL-2, showing that most proliferating cells are not bystander cells. In contrast, the proliferative response to concanavalin A (Con A) was only partially inhibited by treatment of CD4 T cells with GANC, although the efficiency of eliminating IL-2-producing cells was shown to be comparable with that achieved using SEA. This suggests that a portion of the proliferative response to Con A occurs via an alternative pathway not requiring IL-2 synthesis and release.

© 1993 Rockefeller University Press
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The selective ablation of interleukin 2-producing cells isolated from transgenic mice.
L E Minasi, Y Kamogawa, S Carding, K Bottomly, R A Flavell
Journal of Experimental Medicine May 1993, 177 (5) 1451-1459; DOI: 10.1084/jem.177.5.1451

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The Journal of Experimental Medicine: 215 (4)

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April 2, 2018
Volume 215, No. 4

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