Skip to main content

Main menu

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA

User menu

  • Log in

Search

  • Advanced search
JEM
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA
  • Log in
JEM

Advanced Search

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions

You are here

jem Home » 1991 Archive » 1 April » 173 (4): 889
Article

Granulocyte colony-stimulating factor gene transfer suppresses tumorigenicity of a murine adenocarcinoma in vivo.

M P Colombo, G Ferrari, A Stoppacciaro, M Parenza, M Rodolfo, F Mavilio, G Parmiani
M P Colombo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G Ferrari
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A Stoppacciaro
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M Parenza
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M Rodolfo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F Mavilio
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G Parmiani
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1084/jem.173.4.889 | Published April 1, 1991
  • Article
  • Info
  • Metrics
  • Preview PDF
Loading

Abstract

We have investigated the effect of granulocyte colony-stimulating factor (G-CSF) delivery at the site of tumor growth by transducing, via retroviral vector, the human (hu) G-CSF gene into the colon adenocarcinoma C-26 and assaying the ability of transduced cells to form tumors when injected into syngeneic mice. As a control, the same tumor cells were infected with retroviruses engineered to transduce an unrelated gene, the human nerve growth factor receptor, or carry the neomycin resistance gene only. Only cells transduced with the huG-CSF were unable to develop tumors, although huG-CSF was expressed and produced at low level as estimated by both RNA analysis and enzyme-linked immunosorbent assay, indicating that G-CSF can exert an antitumor effect at a physiological dose. Implication of G-CSF as mediator of tumor inhibition was proven by reversing the nontumorigenic phenotype of G-CSF-expressing cells with anti-huG-CSF monoclonal antibody injected at the tumor site. No tumors were formed by injecting C-26 infected cells into nu/nu mice, while neoplastic nodules appeared after injection into sublethally irradiated mice; such tumors, however, regressed when mice normalized their leukocyte counts after irradiation. Tumors were also formed after injection of a mixture of infected and uninfected C-26 cells, although critical delay in tumor formation occurred when infected cells were 10 times more represented in the mixture. Histological examination of tissues surrounding the site of injection showed infiltration of neutrophilic granulocytes, whose number correlated with that of G-CSF-expressing C-26 cells in the injected mixture. These results indicate that G-CSF may have a potent antitumoral activity when released, even at low doses, at the tumor site. The antitumoral effect is mediated by recruitment and targeting of neutrophilic granulocytes to G-CSF-releasing cells.

© 1991 Rockefeller University Press
Previous articleNext article
Back to top
Download PDF
Citation Tools
Granulocyte colony-stimulating factor gene transfer suppresses tumorigenicity of a murine adenocarcinoma in vivo.
M P Colombo, G Ferrari, A Stoppacciaro, M Parenza, M Rodolfo, F Mavilio, G Parmiani
Journal of Experimental Medicine Apr 1991, 173 (4) 889-897; DOI: 10.1084/jem.173.4.889

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts
Sign In to Email Alerts with your Email Address

Email logo Twitter logo Facebook logo Mendeley logo Reddit logo CiteULike logo LinkedIn logo
The Journal of Experimental Medicine: 215 (4)

Current Issue

April 2, 2018
Volume 215, No. 4

  • Table of Contents
  • All Issues

Jump To

  • Article
  • Info
  • Metrics
  • Preview PDF
 

ARTICLES

  • Current Issue
  • Newest Articles
  • Archive
  • Alerts
  • RSS feeds

FOR AUTHORS

  • Submit a Manuscript
  • Instructions for Authors

ABOUT

  • About JEM
  • Editors & Staff
  • Policies & Permissions
  • Advertise
  • Contact Us
  • Feedback
  • Newsroom

CONNECT WITH JEM

  • Email
  • Facebook
  • Twitter
  • RSS
  • Instagram

Online ISSN: 1540-9538
Print ISSN: 0022-1007

Copyright © 2018 JEM by Rockefeller University Press