Expression of the Fc receptor for IgE (Fc epsilon R) was analyzed on alveolar macrophages (AM) in 10 patients with extrinsic allergic alveolitis (EAA) compared with 10 patients with sarcoidosis and to 6 apparently healthy controls. By using the anti-Fc epsilon RII mAb M-L25 in immunocytochemistry experiments, we found that greater than 60% of AM in 10 of 10 patients with EAA were strongly positive, as evidenced by visual analysis in light microscopy and by cytometry. By contrast, no significant staining was detected in sarcoidosis or in controls with either method. Similar results were obtained when Fc epsilon R were identified with preformed immune complexes consisting of NIP-specific human/mouse chimeric IgE antibody plus NIP-ovalbumin. Furthermore, greater than 60% of AM in patients with EAA stained positive for IgE, demonstrating that endogenous IgE is bound to the AM. Our data suggest that IgE antibodies bound to Fc epsilon RII on AM may be involved in pathophysiology of extrinsic allergic alveolitis by activation of the AM after binding of allergen to the cell surface IgE. Furthermore, with the clearcut pattern of Fc epsilon RII expression in extrinsic allergic alveolitis it may be possible to use CD23 antibodies for differential diagnosis of inflammatory lung disease.
Article|
April 01 1990
Expression of the Fc-receptor for IgE (Fc epsilon RII, CD23) on alveolar macrophages in extrinsic allergic alveolitis.
A Pforte,
A Pforte
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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G Breyer,
G Breyer
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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J C Prinz,
J C Prinz
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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P Gais,
P Gais
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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G Burger,
G Burger
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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K Häussinger,
K Häussinger
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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E P Rieber,
E P Rieber
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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E Held,
E Held
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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H W Ziegler-Heitbrock
H W Ziegler-Heitbrock
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
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A Pforte
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
G Breyer
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
J C Prinz
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
P Gais
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
G Burger
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
K Häussinger
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
E P Rieber
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
E Held
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
H W Ziegler-Heitbrock
Medical Department Innenstadt, University of Munich, Federal Republic of Germany.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 171 (4): 1163–1169.
Citation
A Pforte, G Breyer, J C Prinz, P Gais, G Burger, K Häussinger, E P Rieber, E Held, H W Ziegler-Heitbrock; Expression of the Fc-receptor for IgE (Fc epsilon RII, CD23) on alveolar macrophages in extrinsic allergic alveolitis.. J Exp Med 1 April 1990; 171 (4): 1163–1169. doi: https://doi.org/10.1084/jem.171.4.1163
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