Skip to main content

Main menu

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA

User menu

  • Log in

Search

  • Advanced search
JEM
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA
  • Log in
JEM

Advanced Search

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions

You are here

jem Home » 1990 Archive » 1 April » 171 (4): 1101
Article

Strong T cell tolerance in parent----F1 bone marrow chimeras prepared with supralethal irradiation. Evidence for clonal deletion and anergy.

E K Gao, D Lo, J Sprent
E K Gao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D Lo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Sprent
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1084/jem.171.4.1101 | Published April 1, 1990
  • Article
  • Info
  • Metrics
  • Preview PDF
Loading

Abstract

T cell tolerance induction was examined in long-term H-2-heterozygous parent----F1 chimeras prepared with supralethal irradiation (1,300 rad). Although these chimeras appeared to be devoid of host-type APC, the donor T cells developing in the chimeras showed marked tolerance to host-type H-2 determinants. Tolerance to the host appeared to be virtually complete in four assay systems: (a) primary mixed lymphocyte reactions (MLR) of purified lymph node (LN) CD8+ cells (+/- IL-2); (b) primary MLR of CD4+ (CD8-) thymocytes; (c) skin graft rejection; and (d) induction of lethal graft-vs.-host disease by CD4+ cells. Similar tolerance was observed in chimeras given double irradiation. The only assay in which the chimera T cells failed to show near-total tolerance to the host was the primary MLR of post-thymic CD4+ cells. In this assay, LN CD4+ cells regularly gave a significant antihost MLR. The magnitude of this response was two- to fourfold less than the response of normal parental strain CD4+ cells and, in I-E(-)----I-E+ chimeras, was paralleled by approximately 70% deletion of V beta 11+ cells. Since marked tolerance was evident at the level of mature thymocytes, tolerance induction in the chimeras presumably occurred in the thymus itself. The failure to detect host APC in the thymus implies that tolerance reflected contact with thymic epithelial cells (and/or other non-BM-derived cells in the thymus). To account for the residual host reactivity of LN CD4+ cells and the incomplete deletion of V beta 11+ cells, it is suggested that T cell contact with thymic epithelial cells induced clonal deletion of most of the host-reactive T cells but spared a proportion of these cells (possibly low affinity cells). Since these latter cells appeared to be functionally inert in the thymus (in contrast to LN), we suggest that the thymic epithelial cells induced a temporary form of anergy in the remaining host-reactive thymocytes. This anergic state disappeared when the T cells left the thymus and reached LN.

© 1990 Rockefeller University Press
Previous articleNext article
Back to top
Download PDF
Citation Tools
Strong T cell tolerance in parent----F1 bone marrow chimeras prepared with supralethal irradiation. Evidence for clonal deletion and anergy.
E K Gao, D Lo, J Sprent
Journal of Experimental Medicine Apr 1990, 171 (4) 1101-1121; DOI: 10.1084/jem.171.4.1101

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts
Sign In to Email Alerts with your Email Address

Email logo Twitter logo Facebook logo Mendeley logo Reddit logo CiteULike logo LinkedIn logo
The Journal of Experimental Medicine: 215 (4)

Current Issue

April 2, 2018
Volume 215, No. 4

  • Table of Contents
  • All Issues

Jump To

  • Article
  • Info
  • Metrics
  • Preview PDF
 

ARTICLES

  • Current Issue
  • Newest Articles
  • Archive
  • Alerts
  • RSS feeds

FOR AUTHORS

  • Submit a Manuscript
  • Instructions for Authors

ABOUT

  • About JEM
  • Editors & Staff
  • Policies & Permissions
  • Advertise
  • Contact Us
  • Feedback
  • Newsroom

CONNECT WITH JEM

  • Email
  • Facebook
  • Twitter
  • RSS
  • Instagram

Online ISSN: 1540-9538
Print ISSN: 0022-1007

Copyright © 2018 JEM by Rockefeller University Press