Rat T cells and thymocytes were induced to proliferate by a pair of mAbs, MRC OX-54 and MRC OX-55, directed against rat CD2. Accessory cells were required but their role was not simply for crosslinking of the two mAbs, as neither MRC OX-54 nor MRC OX-55 alone, in the presence of a crosslinking second antibody, caused T cell mitogenesis. Nor could the phorbol ester PMA replace either antibody. The two mAbs recognized distinct epitopes on rat CD2; however, MRC OX-54 could partially block MRC OX-55 binding whereas the reverse situation was not seen. A further CD2 epitope was recognized by two mutually competitive mAbs, MRC OX-34 and MRC OX-53, which were not mitogenic. Neither MRC OX-34 nor MRC OX-53 affected the binding of MRC OX-54 or MRC OX-55, yet they prevented the mitogenic effect induced by these mAbs. The presence of mAbs against CD4 and the IL-2-R also abrogated this mitogenesis, whereas an anti-CD5 mAb augmented the CD2-induced proliferation.
Article|
June 01 1988
Activation of rat T lymphocytes by anti-CD2 monoclonal antibodies.
S J Clark,
S J Clark
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
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D A Law,
D A Law
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
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D J Paterson,
D J Paterson
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
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M Puklavec,
M Puklavec
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
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A F Williams
A F Williams
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
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S J Clark
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
D A Law
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
D J Paterson
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
M Puklavec
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
A F Williams
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1988) 167 (6): 1861–1872.
Citation
S J Clark, D A Law, D J Paterson, M Puklavec, A F Williams; Activation of rat T lymphocytes by anti-CD2 monoclonal antibodies.. J Exp Med 1 June 1988; 167 (6): 1861–1872. doi: https://doi.org/10.1084/jem.167.6.1861
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