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jem Home » 1987 Archive » 1 July » 166 (1): 163
Article

Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.

D H Schulze, G Kelsoe
D H Schulze
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G Kelsoe
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DOI: 10.1084/jem.166.1.163 | Published July 1, 1987
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Abstract

The filter paper disc method for cloning inducible lymphocytes was used to census the splenic B cell population of C57BL/6 and BALB/c mice for the expression of three VH gene-families, VH X-24, -Q52, and -J558. B cell colonies, arising from single founder lymphocytes, were identified by in situ hybridization with VH family- and C mu-specific cDNA probes. Some 6.7 X 10(4) C mu+ colonies were screened. Among C57BL/6- or BALB/c-derived colonies, approximately 3% were VH X-24+, approximately 19% were VH Q52+, and approximately 54% were VH J558+. These frequencies are consistent with a process of equiprobable expression for individual VH segments, and provide direct evidence that normal splenic B lymphocytes use a process of random genetic combinatorics to generate the antibody repertoire.

© 1987 Rockefeller University Press
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Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.
D H Schulze, G Kelsoe
Journal of Experimental Medicine Jul 1987, 166 (1) 163-172; DOI: 10.1084/jem.166.1.163

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The Journal of Experimental Medicine: 215 (4)

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April 2, 2018
Volume 215, No. 4

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