Skip to main content

Main menu

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA

User menu

  • Log in

Search

  • Advanced search
JEM
  • Rockefeller University Press
  • JCB
  • JEM
  • JGP
  • LSA
  • Log in
JEM

Advanced Search

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Archive
    • Subject Collections
    • Meeting Collections
  • Reviews & Opinions
    • Editorials
    • Insights
    • Perspectives
    • Reviews
  • Alerts
  • About
    • History
    • Editors & Staff
    • Policies & Permissions
    • Advertise
    • Contact Us
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Publication Fees
    • Author Services
  • Subscriptions

You are here

jem Home » 1986 Archive » 1 March » 163 (3): 550
Article

High-affinity receptor-mediated internalization and degradation of interleukin 2 in human T cells.

M Fujii, K Sugamura, K Sano, M Nakai, K Sugita, Y Hinuma
M Fujii
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
K Sugamura
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
K Sano
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M Nakai
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
K Sugita
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Y Hinuma
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1084/jem.163.3.550 | Published March 1, 1986
  • Article
  • Info
  • Metrics
  • Preview PDF
Loading

Abstract

Receptor-mediated internalization and degradation of IL-2 were investigated in cell lines carrying human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I) and PHA-treated normal PBL. The HTLV-I-carrying cell lines ILT-Yan and TL-Mor, and the PBL expressed both high- and low-affinity IL-2-R. However, another HTLV-I-carrying T cell line, MT-1, expressed mainly low-affinity receptors. Greater than 50% of the IL-2 bound to high-affinity receptors was internalized within 10 min when these cells were incubated at 37 degrees C. The internalized IL-2 was rapidly degraded and the products were excreted into the culture fluid. The t1/2 of IL-2 degradation in these cells was estimated as 60-80 min at 37 degrees C. The internalization and degradation of IL-2 were both temperature dependent. Light-microscopic autoradiography with 3H-labeled IL-2 confirmed the internalization of IL-2, and suggested that some IL-2 might be carried to the nucleus.

© 1986 Rockefeller University Press
Previous articleNext article
Back to top
Download PDF
Citation Tools
High-affinity receptor-mediated internalization and degradation of interleukin 2 in human T cells.
M Fujii, K Sugamura, K Sano, M Nakai, K Sugita, Y Hinuma
Journal of Experimental Medicine Mar 1986, 163 (3) 550-562; DOI: 10.1084/jem.163.3.550

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts
Sign In to Email Alerts with your Email Address

Email logo Twitter logo Facebook logo Mendeley logo Reddit logo CiteULike logo LinkedIn logo
The Journal of Experimental Medicine: 215 (4)

Current Issue

April 2, 2018
Volume 215, No. 4

  • Table of Contents
  • All Issues

Jump To

  • Article
  • Info
  • Metrics
  • Preview PDF
 

ARTICLES

  • Current Issue
  • Newest Articles
  • Archive
  • Alerts
  • RSS feeds

FOR AUTHORS

  • Submit a Manuscript
  • Instructions for Authors

ABOUT

  • About JEM
  • Editors & Staff
  • Policies & Permissions
  • Advertise
  • Contact Us
  • Feedback
  • Newsroom

CONNECT WITH JEM

  • Email
  • Facebook
  • Twitter
  • RSS
  • Instagram

Online ISSN: 1540-9538
Print ISSN: 0022-1007

Copyright © 2018 JEM by Rockefeller University Press