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jem Home » 1978 Archive » 1 September » 148 (3): 746
Article

Alteration of some functional and metabolic characteristics of resident mouse peritoneal macrophages by lymphocyte mediators.

J K Lazdins, A L Kühner, J R David, M L Karnovsky
J K Lazdins
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A L Kühner
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J R David
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M L Karnovsky
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DOI: 10.1084/jem.148.3.746 | Published September 1, 1978
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Abstract

Resident mouse peritoneal macrophages were incubated in Sephadex G-100 fractions of supernates from concanavalin A-stimulated lymphocytes. A significant effect of the lymphocyte supernatant fractions containing mediators on macrophage 5'-nucleotidase, glucose-1 14C oxidation, cell maintenance, and migration is reported. The 5'-nucleotidase was depressed to an extent similar to that seen in activated macrophages obtained from Listeria-infected mice. On the other hand, glucose-1-14C oxidation was enhanced, but not to the same degree as seen in the counterparts in vivo. Whereas migration inhibitory factor (MIF) and cell adherence-augmenting activity were found in a number of adjacent fractions, the metabolic effects were found predominantly in a single fraction. Resident peritoneal macrophages or those elicited by the injection of a lymphocyte-derived chemotactic factor were more responsive with respect to the biochemical changes than caseinate-elicited macrophages. On the other hand, caseinate-elicited macrophages appeared to be more sensitive with respect to the effects of mediator(s) on cell retention. A possible dissociation between MIF and cell-adherence augmenting activity, on the one hand, and the entities that stimulate glucose-1-14C oxidation is reported, based on fractionation studies, and loss of the latter activity upon storage of lymphocyte supernates.

© 1978 Rockefeller University Press
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Alteration of some functional and metabolic characteristics of resident mouse peritoneal macrophages by lymphocyte mediators.
J K Lazdins, A L Kühner, J R David, M L Karnovsky
Journal of Experimental Medicine Sep 1978, 148 (3) 746-758; DOI: 10.1084/jem.148.3.746

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The Journal of Experimental Medicine: 215 (4)

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April 2, 2018
Volume 215, No. 4

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Print ISSN: 0022-1007

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