The idiotype present on the Fab of a phosphorylcholine-binding IgA myeloma protein TEPC 15 (T15) of BALB/c origin was found in normal serum of BALB/c mice. Molecules carrying the T15 idiotype in normal serum could be adsorbed with Sepharose phosphorylcholine beads and R36A pneumococci. The T15 idiotype is absent in germ-free BALB/c but appears when the mice are conventionalized. A survey of normal sera of inbred strains for the T15 idiotype showed it to be present in BALB/c, 129, C57L, C58, and ST and absent or in low levels in CBA, C3H, C57BL/6, C57BL/Ka, C57BL/10, SJL, B10.D2, DBA/2, RIII, A, AL, AKR, NZB, and NH inbred strains of mice. The T15 idiotype is associated with some but not all strains carrying the IgCH allotypes found in BALB/c. Linkage of genes controlling the T15 idiotype in normal serum to the IgCH locus of BALB/c was demonstrated in F2 progeny of a BALB/c and C57BL cross, Bailey's recombinant inbred strains, C x BD, C x BE, C x BG, C x BH, C x BI, C x BJ, C x BK, and CB20 congenic strains. Among these strains, only those possessing the IgCH locus of BALB/c including the F2 progeny consisting of BALB/c homozygotes and BALB/c/C57BL heterozygotes and C x BG and C x BJ recombinants showed the T15 idiotype.
Article|
April 01 1974
GENETICS OF A NEW IgVH (T15 IDIOTYPE) MARKER IN THE MOUSE REGULATING NATURAL ANTIBODY TO PHOSPHORYLCHOLINE
R. Lieberman,
R. Lieberman
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
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M. Potter,
M. Potter
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
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E. B. Mushinski,
E. B. Mushinski
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
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W. Humphrey, Jr.,
W. Humphrey, Jr.
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
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S. Rudikoff
S. Rudikoff
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
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R. Lieberman
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
M. Potter
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
E. B. Mushinski
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
W. Humphrey, Jr.
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
S. Rudikoff
From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
Received:
January 15 1974
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1974 by The Rockefeller University Press
1974
J Exp Med (1974) 139 (4): 983–1001.
Article history
Received:
January 15 1974
Citation
R. Lieberman, M. Potter, E. B. Mushinski, W. Humphrey, S. Rudikoff; GENETICS OF A NEW IgVH (T15 IDIOTYPE) MARKER IN THE MOUSE REGULATING NATURAL ANTIBODY TO PHOSPHORYLCHOLINE . J Exp Med 1 April 1974; 139 (4): 983–1001. doi: https://doi.org/10.1084/jem.139.4.983
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