Experimental allergic encephalomyelitis (EAE) was induced in rats of various genotypes by injection of 10 µg guinea pig basic protein in complete Freund's adjuvant containing 100 µg H37 RV M. tuberculosis. Histologically verified EAE was present in 20/20 Lewis, 17/17 (Lewis x BN)F1, 9/9 Lewis backcross, and 21/42 BN backcross rats. Among the BN backcross animals, 25/42 were determined to carry the major histocompatibility type characteristic of the Lewis strain and 21 of these had EAE. Separate groups of Lewis, BN, and (Lewis x BN)F1 rats were immunized as described and skin tested on day 13 with 10 µg guinea pig basic protein and rat S basic protein. Animals of each genotype had Arthus and delayed skin reactivity to both antigens. These data are compatible with the hypothesis that susceptibility to EAE in rats is controlled by an autosomal dominant gene linked to the major histocompatibility locus. It is proposed that this is an immune response gene, designated Ir-EAE, which controls T cell reactivity directed against a highly encephalitogenic portion of the basic protein molecule.
Article|
October 01 1973
LINKAGE OF SUSCEPTIBILITY TO EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS TO THE MAJOR HISTOCOMPATIBILITY LOCUS IN THE RAT
R. Michael Williams,
R. Michael Williams
From the Department of Pathology, Harvard Medical School, Department of Neurology Boston University School of Medicine, and Department of Neuroscience, Childrens Memorial Hospital, Boston, Massachusetts 02115
Search for other works by this author on:
Michael J. Moore
Michael J. Moore
From the Department of Pathology, Harvard Medical School, Department of Neurology Boston University School of Medicine, and Department of Neuroscience, Childrens Memorial Hospital, Boston, Massachusetts 02115
Search for other works by this author on:
R. Michael Williams
From the Department of Pathology, Harvard Medical School, Department of Neurology Boston University School of Medicine, and Department of Neuroscience, Childrens Memorial Hospital, Boston, Massachusetts 02115
Michael J. Moore
From the Department of Pathology, Harvard Medical School, Department of Neurology Boston University School of Medicine, and Department of Neuroscience, Childrens Memorial Hospital, Boston, Massachusetts 02115
Received:
June 05 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 138 (4): 775–783.
Article history
Received:
June 05 1973
Citation
R. Michael Williams, Michael J. Moore; LINKAGE OF SUSCEPTIBILITY TO EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS TO THE MAJOR HISTOCOMPATIBILITY LOCUS IN THE RAT . J Exp Med 1 October 1973; 138 (4): 775–783. doi: https://doi.org/10.1084/jem.138.4.775
Download citation file: