Guinea pigs with a genetically determined total deficiency of the fourth component of complement have been studied for various in vivo immunological functions. Passive cutaneous anaphylaxsis, contact and delayed hypersensitivity, and the cellular exudative response to a foreign body were normal. These animals also have normal direct and reverse passive Arthus reactions which suggest that they possess a mechanism to bypass C4 and directly activate late-acting complement components. This would appear to be an unequivocal demonstration of an alternate pathway in the complement sequence. Immune clearance of guinea pig erythrocytes sensitized with rabbit antibody was impaired in the deficient animals. Antibody production in C4-deficient animals was impaired for two of the three antigens studied.
Article|
July 01 1971
IN VIVO STUDIES IN C4-DEFICIENT GUINEA PIGS
Leonard Ellman,
Leonard Ellman
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
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Ira Green,
Ira Green
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
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Francis Judge,
Francis Judge
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
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Michael M. Frank
Michael M. Frank
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
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Leonard Ellman
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
Ira Green
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
Francis Judge
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
Michael M. Frank
From the Laboratory of Immunology and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases; and the Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014
Received:
March 01 1971
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1971 by The Rockefeller University Press
1971
J Exp Med (1971) 134 (1): 162–175.
Article history
Received:
March 01 1971
Citation
Leonard Ellman, Ira Green, Francis Judge, Michael M. Frank; IN VIVO STUDIES IN C4-DEFICIENT GUINEA PIGS . J Exp Med 1 July 1971; 134 (1): 162–175. doi: https://doi.org/10.1084/jem.134.1.162
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