The distribution, and quantity of immunoglobulins on the surface of lymphocytes has been studied by means of immunofluorescence and a quantitative radio-immunoassay. Surface immunoglobulins were found on approximately 45% of spleen and marrow lymphocytes and 7–14% of lymphocytes from lymph nodes, peripheral blood, and peritoneal exudate. Thymic lymphocytes contained undetectable amounts of immunoglobulin. In the spleen the different immunoglobulins were present in the following order: γG2 > γG1 > M > γA > γG3. The surface immunoglobulin was largely removable by brief treatment with trypsin. Quantitative analysis indicated that 50,000–150,000 molecules of immunoglobulin were present on an individual cell. A variety of observations make it likely that this lymphocyte-associated immunoglobulin. is a product of the cell to which it is attached rather than a form of cytophilic antibody.
Article|
January 01 1971
IMMUNOGLOBULINS ON THE SURFACE OF LYMPHOCYTES : I. DISTRIBUTION AND QUANTITATION
Enrique Rabellino,
Enrique Rabellino
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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Sonia Colon,
Sonia Colon
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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Howard M. Grey,
Howard M. Grey
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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Emil R. Unanue
Emil R. Unanue
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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Enrique Rabellino
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Sonia Colon
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Howard M. Grey
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Emil R. Unanue
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Received:
August 27 1970
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1971 by The Rockefeller University Press
1971
J Exp Med (1971) 133 (1): 156–167.
Article history
Received:
August 27 1970
Citation
Enrique Rabellino, Sonia Colon, Howard M. Grey, Emil R. Unanue; IMMUNOGLOBULINS ON THE SURFACE OF LYMPHOCYTES : I. DISTRIBUTION AND QUANTITATION . J Exp Med 1 January 1971; 133 (1): 156–167. doi: https://doi.org/10.1084/jem.133.1.156
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